Extrakce psilocybinu a psilocinu z hub

[G.Patton]

~20-30%

 

[Neo156773]

Do you know if 4-aco-dmt freebase is soluble in ethyl acetate and if yes how many mg/ml ?

and is ethyl acetate a nice solvent to dissolve a mix of psilocin freebase & acetic anhydride (1,1mol e.q) & sodium acetate (0,1 mol e.q) ( acetylation process of psilocin —> 4-aco-dmt)

maybe for better yield performing acetylation process under influence of nitrogen ballon to reduce oxidation and humidity ) -> then after acetylation neutralize tensing acetic anhydride with water to the ethyl acetate (which contains acetic anhydride & Psilocin freebase & sodium acetate Then adding a bit sodium hydroxide to pH 10 and then extracting 4aco dmt freebase again (should hopefully end in ethyl acetate layer/phase). Then drying ethyl acetate with sodium sulfate and then rotary evoporation to obtain 4aco dmt freebase.
Then dissolving in hot acetone (jar 1) und also dissolve the right amount of fumaric acid in acetone (jar 2). Then mixing (jar 1 and 2) and stirr for a few mins on a magnetic stirrer and then rotary evaporation to obtain 4-aco-dmt fumarate ( for long term storage free from humidity/light/oxygen & maybe in the refrigerator ) what do you think ?

 

[G.Patton]

Hello, I don't know for sure about solubility. I assume yes.

 

[G.Patton]

Dobrý den, omlouvám se za dlouhou odpověď. Ano, můžete. Postup je stejný.

 

[Neo156773]

It does not work for amanita
A/B-tek does not work because Muscimol freebase is still not soluble in organic solvents like ethyl acetate.
I tried it.
You can try to extract with 10% acetic acid and then defat with xylene/ethyl acetate.
But basing with ammonia (pH 8-10) and then extracting with organic solvent won’t work

 

[Neo156773]

Nobody said that amanitas contain psilocybin?
he/she asked if it’s possible to do an a/b tek for amanitas ( for Muscimole hydrochloride I guess) and the answer is no

 

[Neo156773]

Muscimole is very polar even as freebase. It will prefer the aqueous layer and not the organic layer (toluene/naphta/xylene/dcm/ethyl acetate).
That’s the problem.
I tried it with xylene and yield was very bad

 

[Osmosis Vanderwaal]

well it was posted in a thread on psilocin/psilocybin extraction . and not everyone is aware of soma and english is his secnd language, so it's likely he didn't realize you were talking about not psilocybiasn mushrooms because that is off-topic in this thread.

 

[Osmosis Vanderwaal]

I'm seeing ethanol and then hydrobromination, which makes the solubility 1mg/ml Muscimole hcl has a sol. of 300mg/ml in water and the base is 11.4mg/ml, so you should come ahead about 280mg/ml when you deprotonate it(280mg could precipitate from aa ml of water)

 

[mycelium]

What is a good amount of ammonium succinate? to put in your medium? In your liquid culture if you're growing active mycelium?

And, can one use DCM or chloroform or heptane, something other than diethyl ether to extract?

 

[Osmosis Vanderwaal]

I've never used nor I have I heard anybody else mention using ammonium succinate, I just always used the easy stuff malt extract from a brew store or boil potatoes and collect the water and add (Karo) cornsyrup for PDA. At the end of the dat adding more nutrients is counterproductive to expanding mass in a liquid culture because you want it to grow linearlly in the search for food, and if it has plenty of food, it doesnt stretch out. Polar solvents like water and alcohols always seemed to get a pretty complete extraction for me