Hello, regarding the refractrometer, since we do not work with it, we do not know how this equipment works.
Regarding the analytical method used for quantitative analysis of enantiomers, we use analytical standards S and R. First of all we use a screen method to identify and quantify the compounds in the sample, then we move to the enantiomers method (LC-MS with chiral column). Then, with a chiral column (chromatography) that is coated with a chiral compound, it interacts differently with the two enantiomers of these substances. This allows the enantiomers to separate as they pass through the column. The mass spectrometer can then be used to quantify the amount of each enantiomer present.